DNA

Part:BBa_K4769304:Design

Designed by: Kieran Abbott   Group: iGEM23_Cambridge   (2023-10-10)


gyrA promoter 3’ integration sequence for B. subtilis 3610


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 642
    Illegal AgeI site found at 254
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 225


Design Notes

N/A


Source

B. subtilis 3610 genome

Primers for cloning: FW: ctagcaatttaatgtgtataatcataagtttattgatataatgg (+ desired overhangs) RV: atctgtgataccctctatctttttgtcc (+ desired overhangs)

References